X-ray observations of RX J0822-4300 and Puppis-A

Based on observations with the X-ray observatories Chandra and XMM-Newton we present results from a detailed spectro-imaging and timing analysis of the central compact X-ray source RX J0822-4300 in the supernova remnant Puppis-A. The superior angular resolution of Chandra allows for the first time to pinpoint the point source nature of this object down to $0.59\pm0.01$ arcsec (FWHM) and to determine its position:{RA=$08^{\rm h}21^{\rm m}57.40^{\rm s}$, Dec=$-43^{\circ}00^{'}16.69^{''}$ (J2000)} with sub-arcsecond accuracy. Spectral fits based on Chandra and XMM-Newton data provide a tight constraint on the emission properties of RX J0822-4300. Most of its X-ray emission seems to be of thermal origin. A model spectrum consisting of two blackbody components with $T_{1}\simeq 2.6\times10^{6}$ K, $T_{2}\simeq 5.0\times10^{6}$ K and $R_{1}\simeq 3.3$ km, $R_2 \simeq 0.75$ km for the blackbody temperatures and the size of the projected emitting regions, respectively, provides the best model description of its spectrum. A search for X-ray pulsations from RX J0822-4300, revealed an interesting periodicity candidate which, if confirmed, does not support a scenario of steady spin-down.Comment: Accepted by A&A, 20 pages, 10 figures, 3 table

Nanobody-dependent delocalization of endocytic machinery in Arabidopsis root cells dampens their internalization capacity

Plant cells perceive and adapt to an ever-changing environment by modifying their plasma membrane (PM) proteome. Whereas secretion deposits new integral membrane proteins, internalization by endocytosis removes membrane proteins and associated ligands, largely with the aid of adaptor protein complexes and the scaffolding molecule clathrin. Two adaptor protein complexes function in clathrin-mediated endocytosis at the PM in plant cells, the heterotetrameric Adaptor Protein 2 (AP-2) complex and the octameric TPLATE complex (TPC). Whereas single subunit mutants in AP-2 develop into viable plants, genetic mutation of a single TPC subunit causes fully penetrant male sterility and silencing single subunits leads to seedling lethality. To address TPC function in somatic root cells, while minimizing indirect effects on plant growth, we employed nanobody-dependent delocalization of a functional, GFP-tagged TPC subunit, TML, in its respective homozygous genetic mutant background. In order to decrease the amount of functional TPC at the PM, we targeted our nanobody construct to the mitochondria and fused it to TagBFP2 to visualize it independently of its bait. We furthermore limited the effect of our delocalization to those tissues that are easily accessible for live-cell imaging by expressing it from the PIN2 promotor, which is active in root epidermal and cortex cells. With this approach, we successfully delocalized TML from the PM. Moreover, we also show co-recruitment of TML-GFP and AP2A1-TagRFP to the mitochondria, suggesting that our approach delocalized complexes, rather than individual adaptor complex subunits. In line with the specific expression domain, we only observed minor effects on root growth and gravitropic response, yet realized a clear reduction of endocytic flux in epidermal root cells. Nanobody-dependent delocalization in plants, here exemplified using a TPC subunit, has the potential to be widely applicable to achieve specific loss-of-function analysis of otherwise lethal mutants

Role of finite layer thickness in spin-polarization of GaAs 2D electrons in strong parallel magnetic fields

We report measurements and calculations of the spin-polarization, induced by a parallel magnetic field, of interacting, dilute, two-dimensional electron systems confined to GaAs/AlGaAs heterostructures. The results reveal the crucial role the non-zero electron layer thickness plays: it causes a deformation of the energy surface in the presence of a parallel field, leading to enhanced values for the effective mass and g-factor and a non-linear spin-polarization with field.Comment: 4 pages, 4 figures, Fig. 4 has been replaced from the previous version, minor changes in the tex

Anomalous Rashba spin splitting in two-dimensional hole systems

It has long been assumed that the inversion asymmetry-induced Rashba spin splitting in two-dimensional (2D) systems at zero magnetic field is proportional to the electric field that characterizes the inversion asymmetry of the confining potential. Here we demonstrate, both theoretically and experimentally, that 2D heavy hole systems in accumulation layer-like single heterostructures show the opposite behavior, i.e., a decreasing, but nonzero electric field results in an increasing Rashba coefficient.Comment: 4 pages, 3 figure

Discovery of a 112 ms X-ray Pulsar in Puppis A: Further Evidence of Neutron Stars Weakly Magnetized at Birth

We report the discovery of 112-ms X-ray pulsations from RX J0822-4300, the compact central object (CCO) in the supernova remnant Puppis A, in two archival Newton X-Ray Multi-Mirror Mission observations taken in 2001. The sinusoidal light curve has a pulsed fraction of 11% with an abrupt 180 deg. change in phase at 1.2 keV. The observed phase shift and modulation are likely the result of emission from opposing thermal hot spots of distinct temperatures. Phase-resolved spectra reveal an emission feature at E(line) = 0.8 keV associated with the cooler region, possibly due to an electron cyclotron resonance effect similar to that seen in the spectrum of the CCO pulsar 1E 1207.4-5209. No change in the spin period of PSR J0821-4300 is detected in 7 months, with a 2 sigma upper limit on the period derivative less than 8.3E-15. This implies limits on the spin-down energy loss rate of less than 2.3E35 erg/s, the surface magnetic dipole field strength B_s < 9.8E11 G, and the spin-down age tau > 220 kyr. The latter is much longer than the SNR age, indicating that PSR J0821-4300 was born spinning near its present period. Its properties are remarkably similar to those of the two other known CCO pulsars, demonstrating the existence of a class of neutron stars born with weak magnetic fields related to a slow original spin. These results are also of importance in understanding the extreme transverse velocity of PSR J0821-4300, favoring the hydrodynamic instability mechanism in the supernova explosion.Comment: 5 pages, 4 figure, Latex, emulateapj style. To appear in the Astrophysical Journa

The Effect of Spin Splitting on the Metallic Behavior of a Two-Dimensional System

Experiments on a constant-density two-dimensional hole system in a GaAs quantum well reveal that the metallic behavior observed in the zero-magnetic-field temperature dependence of the resistivity depends on the symmetry of the confinement potential and the resulting spin-splitting of the valence band

LA RIFORMA FRANCESE DEL DIRITTO DELL’ARBITRATO: UN COMMENTO SISTEMATICO. PARTE SECONDA. IL LODO, LE IMPUGNAZIONI E LA DISCIPLINA DELL’ARBITRATO INTERNAZIONALE

Commento sistematico al decreto francese del 2011riformatore della materia arbitrale